Reclamation Information Sharing Environment (RISE)

Early detection of invasive mussels remains a critical priority for the Bureau of Reclamation, as rapid establishment can cause severe operational and economic impacts. Current laboratory methods rely on microscopy for detection of veliger larvae collected from plankton tows and concentrated water samples. While effective, these methods are labor-intensive and sensitive to inefficiencies in sample handling and subsample collection. Four main aspects of sample handling and detection were examined: 1) optimization of subsample collection after sample settlement, 2) review of external laboratory SOP’s, 3) exploration of automated imaging technologies, and 4) preliminary evaluation of alternative detection methods based on volatile compound analysis. Relative to current methods, utilizing a modified venoset and 1/4-inch interior diameter tubing at the base of the sample settlement cone, a 3/8-inch interior diameter silicone tubing reduced cone construction and repair needs, improved setup and takedown efficiency, decreased spill frequency, and significantly lowered material and labor cost. Scoping investigations into automated imagery of samples and detection of veliger scent in samples found promising results and leads that warrant further investigation. Initial studies have shown that canines can detect adult mussels and veligers in plankton tow samples containing ethanol and electronic scent detection technologies should be a focus of future testing.