Catalog Item

S&T Project 19006 Final Report: Development of Dreissenid Mussel Engineered Disseminated Neoplasia

Biomilab LLC has been working for three years on a project aimed at developing, validating, and manufacturing an invasive mussel eradication agent that mimics a natural pathogenic mechanism found in marine bivalves called a disseminated neoplasia (DN). A DN is a form of cancer where the cancer cells themselves travel between organisms in a specific-species manner causing toxicity in the host. Biomilab has established invasive mussel aquaculture in their laboratory and determined methods for the prolonged survival of dissociated mussel cells in culture. They have utilized sequence data provided by Reclamation, and plasmid DNA vectors were constructed for transgene expression and tested in insect cell lines as a proxy for mussel cells. Biomilab tested more than a dozen methods of transduction on mussel cells and determined that because mitotic cells are quite rare, transduced foreign DNA cannot enter the nucleus and is not expressed. In the third year, the focus was on overcoming issues of transduction by exploring infection with recombinant viruses and by establishing methods for the controlled spawning and generation of quagga and zebra mussel embryos by in vitro fertilization.
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Generation Effort S&T Project 19006: Development of Dreissenid Mussel Engineered Disseminated Neoplasia
Location Name Western US
Type Uploaded file(s)
File Type PDF
Publisher Bureau of Reclamation
Publication Date Friday, September 30th, 2022
Update Frequency not planned
Last Update Thursday, September 22nd, 2022

Disclaimer

The findings and conclusions of this work are those of the author(s) and do not necessarily represent the views of the Bureau of Reclamation.