Reclamation Information Sharing Environment (RISE)

The current project developed a high-quality, chromosome-scale assembly of the quagga mussel genome as a resource for the development of biocontrols and investigations of quagga mussel biology. Two methods of high-throughput DNA sequencing were used to maximize both completeness and the accuracy of genome sequencing. Multiple different bioinformatic approaches were tested to optimize the genome assembly. Scaffolding of the assembly was complemented using Hi-C chromosomal conformation analysis, which facilitated reconstruction of chromosome-scale pseudomolecules. RNA sequencing and transcript assembly was also conducted to allow identification of genes of interest and to facilitate ongoing efforts to annotate the genome.