Catalog Record

Report From S&T Project 20026: Investigation of Environmental RNA (eRNA) as a Detection Method for Dreissenid Mussels and Other Invasive Species

In the last ten years, environmental DNA (eDNA) has emerged as a technique for the detection and monitoring of invasive, endangered, and threaten species. The use of environmental RNA (eRNA) is starting to emerge as a method for addressing questions that eDNA cannot answer. eRNA could be a useful tool in determining if an organism is alive or dead. The goal of this project was to investigate eRNA preservation and extraction methods for the detection of the quagga mussel (QM) 16S and 18S transcripts. eRNA samples were collected at Canyon Reservoir, AZ, preserved three different ways, and three different RNA extraction kits were used. RT-qPCR was performed on the samples for both the 16S and 18S transcripts. It was possible to detect the 18S transcript with all three-preservation methods and the three extraction methods. The 16S transcript was not detected when preserved with the RNAlater preservative and with the Zymo Water and Monarch Kits. The project showed that it is possible to extract viable eRNA from water samples. Additional research is needed to optimize the collection, extraction, and analysis methods for eRNA, and to determine the best uses of eRNA in the early detection and management of invasive species.
Generation Effort S&T Project 20026: Investigation of Environmental RNA (eRNA) as a Detection Method for Dreissenid Mussels and Other Invasive Species
Themes Biological
Tags Quagga Mussels, Dreissenid Mussel, Environmental DNA (eDNA), Zebra Mussel, Environmental Ribonucleic Acid (eRNA)
Reclamation Project
Reclamation Program Science and Technology Program

Location Information

Location Description The Bureau of Reclamation’s Technical Service Center (TSC) provides innovative solutions to water and power resource issues. The TSC consists of approximately 565 scientists, engineers, and other professional and office staff, and operates a world-class materials and hydraulic engineering laboratory. TSC's mission is to be Reclamation’s preferred provider of science and engineering services, and to be the preferred employer of engineers and scientists in the U.S. TSC values are founded on personal and professional integrity. The TSC is located at the Denver Federal Center, 6th and Kipling, Building 67, Denver, Colorado 80225-0007.
Location Tags Denver Federal Center
Location Parent
State(s) Colorado
Unified Region(s) Upper Colorado Basin
Timezone MT
Elevation 5,619 ft
Vertical Datum NAVD88
Coordinates (lat, long) See Location Details
Horizontal Datum WGS84