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Report from S&T Project 19009: Evaluating the Sensitivity and Reproducibility of Environmental DNA Field Sampling Workflows for Detection of Dreissenid Mussels

There is a need to develop and evaluate eDNA dreissenid mussel field sampling protocols. We compared two common eDNA field sampling methods, sub-surface grab samples and plankton tow samples, in 12 waterbodies with known or potential dreissenid mussel infestations spanning the coterminous United States. To mimic early detection sampling scenarios, we sampled waterbodies or areas within waterbodies that had lower densities of dreissenid mussels or were suspect for dreissenid mussels. The two eDNA sampling methods resulted in similar dreissenid mussel DNA detection patterns. Both methods detected target DNA at the same 7 waterbodies and both methods failed to detect target DNA at the same 5 waterbodies. At one of the lower density sites, more mussel DNA was amplified in plankton tow samples than from grab samples.
Generation Effort S&T Project 19009: Evaluating the Sensitivity and Reproducibility of Environmental DNA Field Sampling Workflows for Detection of Dreissenid Mussels
Location Western US
Themes Environmental
Tags Quagga Mussels, Zebra Mussels, Detection, Environmental DNA (eDNA), Dreissenid Mussels
Reclamation Project
Reclamation Program Science and Technology Program

Location Information

Location Name Western US
Location Description The 17 western states of Washington, Oregon, Idaho, Montana, North Dakota, South Dakota, Nebraska, Wyoming, California, Nevada, Utah, Colorado, Kansas, Oklahoma, Texas, New Mexico, and Arizona..
Location Tags Reclamation Domain
Location Parent
State(s) Arizona, California, Colorado, Idaho, Kansas, Montana, North Dakota, Nebraska, New Mexico, Nevada, Oklahoma, Oregon, South Dakota, Texas, Utah, Washington, Wyoming
Unified Region(s) Missouri Basin, Arkansas-Rio Grande-Texas-Gulf, Upper Colorado Basin, Lower Colorado Basin, Columbia-Pacific Northwest, California-Great Basin
Timezone MT
Elevation [ N/A ]
Vertical Datum NAVD88
Coordinates (lat, long) See Location Details
Horizontal Datum WGS84